USP14 regulates H2O2 induced oxidative stress in H9c2 cells

AIM:To evaluate the effect of inhibiting ubiquitin-specific protease 14(USPl4) activity on oxidative stress induced by H₂O₂ of H9c2 cells.METHODS:The H9c2 cells were incubated with H₂O₂ at 25 μmol/L for 2 h to establish the oxidative stress injury model.The cells were divided into control group,H₂O₂ group,IU1 group (25 μmol/L or 50 μmol/L) and IU1+H₂O₂ group.The H9c2 cells activity was measured by MTS assay.The level of intracellular reactive oxygen species (ROS) and cell survival rate were analyzed by flow cytometry assay.The changes of the mitogen-activated protein kinase (MAPK) family related proteins were detected by Western blot.RESULTS:Compared with control group,the cell activity and the viability rate in H₂O₂ group were decreased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were increased (P<0.05).Compared with H₂O₂ group,the cell activity and the viability rate of the H9c2 cells in IU1+H₂O₂ group were increased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were decreased (P<0.05).CONCLUSION:Inhibition of USPl4 activity reduces the oxidative stress injury of the H9c2 cells.The mechanism may be related to inhibition of the MAPK signaling and down-regulation of apoptosis related proteins.     

Spatial-Temporal Changes in Grain Production,Consumption and Driving Mechanism in China

The spatial-temporal patterns of grain production and consumption have an important influence on the effective national grain supply on condition of tight balance in the total grain amount in China. In this paper, we analyze the spatial-temporal patterns of grain production, consumption and the driving mechanism for their evolution processes in China. The results indicate that both gravity centers of grain production and consumption in China moved toward the northern and eastern regions, almost in the same direction. The coordination of grain production and consumption increased slightly from 1995 to 2007 but decreased from 2000 to 2007. There is a spatial difference between the major districts of output increase and the strong growth potential in grain consumption, which indicates an increasing difficulty in improving the regional coordination of grain production and consumption. The movement of the gravity center of grain production is significantly correlated with regional differences in grain production policy, different economic development models, and spatial disparity of land and water resource use. For grain consumption, the main driving factors include rapid urbanization, the upgrade of food consumption structure, and distribution of food industries.     

Effect of transketolase-like protein 1 on proliferation of human nasopharyngeal carcinoma cells

AIM: To investigate the effect of transketolase-like protein 1 (TKTL1) on proliferation of human nasopharyngeal carcinoma cells in vitro. METHODS: The siRNA against TKTL1 mRNA was constructed and transfected into human nasopharyngeal carcinoma cells (CNE cell line). The activity of transketolase was detected before and after RNA interference.Real-time PCR was used to determine the mRNA expression of transketolase (TKT) gene family in the CNE cells.Flow cytometry and MTT test were used to detect the effect of anti-TKTL1 siRNA on cell proliferation and cell cycle in the CNE cells. RESULTS: The total transketolase activity was significantly decreased in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector or untransfected CNE cells. No significant difference in the expression level of TKT and TKTL2 gene between the CNE cells transfected with siRNA TKTL1 construct and the cells transfected with control vector or untransfected CNE cells was observed (P>0.05). However, the expression level of TKTL1 gene was significantly downregulated in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector.Cancer cells were arrested in G0/G1 phase, and cancer cell proliferation was significantly inhibited in the CNE cells transfected with siRNA TKTL1 construct. CONCLUSION: TKTL1 plays an important role in the total transketolase activity and cell proliferation of human nasopharyngeal carcinoma. TKTL1 may be considered as a potential target for novel anti-cancer therapy.     

Effect of Beclin 1 RNA interference on Vit K3 injured SMMC-7721 cells

AIM: To observe the effect of Beclin 1 silencing by RNA interference (RNAi) technique to the injury of SMMC-7721 hepatoma cells by vitamin K3 (Vit K3).METHODS: The recombinant plasmid Psilencer 3.1-siRNA-Beclin 1 was transfected into SMMC-7721 hepatoma cells by eukaryotic cell transfection technique. Plasmid vector and cell culture medium were used as negative and control, respectively. The cells were collected 48 h later to extract cell RNA and total protein and to detect Beclin 1 gene expression by RT-PCR and Western blotting. 40 μmol/L Vit K3 was used to treate the Beclin 1-siRNA cells, Hoechst33342 staining was used for the determination of the percentage of cell apoptosis.RESULTS: Compared with the control group, the synthetic siRNA of Beclin 1 significantly decreased the levels of Beclin 1 mRNA and protein expressions. Beclin 1 mRNA was up-regulated in 40 μmol/L Vit K3 treated SMMC-7721 hepatoma cells, the percentage of apoptosis cells increased (P﹤0.01). In beclin 1-siRNA cells, Beclin 1 mRNA was down-regulated obviously, the percentage of apoptosis cells increased significantly compared with the 40 μmol/L Vit K3 group (P﹤0.01).CONCLUSION: The transfection of SMMC-7721 hepatoma cells by Psilencer3.1-siRNA-Beclin 1 effectively inhibits the expressions of Beclin 1 mRNA and protein, inhibits the activation of Beclin 1 dependent autophagic signaling pathway, and aggravates the apoptosis induced by Vit K3.     

沼气发酵过程混合搅拌研究进展

在沼气工程中设置混合搅拌装置,可以提高反应的传质效率,加快产沼气过程的进行,而混合搅拌装置的电耗占整个沼气工程电耗的很大部分,这成为混合搅拌装置深入推广的主要限制因素.本文对国内外沼气发酵混合搅拌过程中搅拌方式和搅拌模式的研究现状进行综述.     

茎芥菜胞质四倍体白菜雄性不育系花药发育的研究

 以茎芥菜胞质雄性不育系与四倍体白菜杂交获得的同源四倍体白菜异源胞质雄性不育系及其保持系为材料，采用形态学和石蜡切片方法研究其花药解剖结构及发育。结果表明：该雄性不育为结构性雄性不育，其退化或畸形雄蕊分为5种类型：盾状雄蕊、条状雄蕊、片状雄蕊、羽状雄蕊和瓣状雄蕊。该雄性不育系花药发育败育有两个时期，盾状雄蕊花药败育于孢原细胞分化期，雄蕊整个发育时期均处在孢原细胞分化期，无绒毡层与花粉母细胞的分化，不形成药室，属孢子体败育型；其它类型雄蕊，花药败育发生在雄蕊原基分化时期，由于雄蕊原基偏离正常的分化轨道，形成瓣状化雄蕊。     

切碎灭茬还田机的研制

国家专利产品4JQM-110型玉米秸秆切碎灭茬还田机,配套动力50-80马力拖拉机,后置双行作业,结构原理独特,利用卧式高速旋转刀,同时完成秸秆切碎和灭茬保墒复式作业,属国内独创.经省级农机专家鉴定,技术属国际领先.灭茬机构模仿人工用钣镢收获玉米秸秆的过程,把砍切刀设计成卧式高速旋转刀,使收获玉米秸秆的过程高速连续化,完成灭茬覆土保墒作业;秸秆切碎机构模仿人工用铡刀铡切玉米秸秆的过程把切刀设计成卧式高速旋转刀,使切碎秸秆过程高速连续化完成秸秆切碎作业.     

轻卡汽车门锁故障分析与解决措施

门锁的定义为锁止车门的机构,主要包括锁体、挡块、内外操纵机构和内外锁止机构,是驾驶室的保护安全部件.本文介绍了轻卡汽车门锁常见故障.分析了门锁失效原因,提出了对轻卡汽车门锁常见问题的解决措施.     

蓝狐精子受精能力的研究——体外常温保存精子存活率的检测

本试验对３０ 只进口芬兰大体型种公狐的１１２ 份精液于１９９８ 和１９９９ 年度的两个繁殖季节进行了精子存活率的检测。结果表明：进口的芬兰大体型种公狐人工授精的可利用率为８３－４ ％ ；精子的平均总存活时间分别为Ａ 组２６－０ ｈ ，Ｂ 组２１－７ ｈ，Ｃ 组５ ｈ ；精子的平均生存指数为Ａ 组１６－６ ，Ｂ 组１０－８ ，Ｃ 组０－８ 。     

基于表型值和育种值的中国对虾生长、抗逆性状相关分析

利用单性状动物模型估计中国对虾170d的体重、抗WSSV存活时间和存活率3个性状的育种值,在家系水平上进行性状间表型值和育种值相关分析。估计的3个性状的遗传力分别为:0.22±0.16、0.14±0.12和0.03±0.021,并据此计算各性状个体育种值。家系性状表型值的相关分析表明,家系170d体重和抗WSSV存活时间之间存在一定程度相关(r=0.35,P<0.05),其余性状间相关系数很小,且差异不显著。3个性状家系育种值间的相关系数均较小,170d体重与抗WSSV存活时间的相关系数最高(0.038),170d体重与存活率,抗WSSV存活时间与存活率之间为负相关(r=-0.24,r=-0.027),并且统计检验未达到显著性水平。研究结果表明,利用多性状复合育种技术,培育综合性状优良的中国对虾新品种是一个正确而必要的育种策略。